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KMID : 1005220070220020137
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Journal of Embryo Transfer
2007 Volume.22 No. 2 p.137 ~ p.141
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Chromosomal Analysis of Hanwoo Embryos by In Vitro Culture Condition
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Choi Sun-Ho
Cho Sang-Rae
Han Man-Hye
Kim Hyun-Jong
Choe Chang-Yong
Son Dong-Soo
Kim Sang-Keun
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Abstract
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Antioxidants were well known to be essential supplements in the complex media and serve as a reservoir of oxygen. In this study, Hanwoo COCs (cumulus oocytes complexes) were matured and developed in L-cysteine-TCM199 and analyzed metaphase chromosome. Maturation rate of Hanwoo COCs were 73.4%, 94.6% in 0.1% PVA, 0.1 mM L-cysteine, respectively and showed significantly different between the treatments (p<0.05). Blastocyst formation were revealed 20.3%, 10.0% in 5% FBS+TCM199, 0.1 mM L-cysteine+1% BSA, respectively. There were no significant difference among treatment groups. Metaphase chromosome were showed 18.3%, 12.0% in 5% FBS-TCM199, 0.1 mM L-cysteine, respectively and analyzable chromosome were 6.1%, 4.0% and had no differences between the treated groups. In the case of in vitro developmental stages, metaphase chromosome were showed 18.3%, 12.0% in $4{\sim}16$ cells stage, 43.1%, 13.0% in morulae stage and 94.8%, 100.0% in blastocyst stage. These results suggested L-cysteine has beneficial role for in virto maturation and development in Hanwoo COCs.
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KEYWORD
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in vitro, embryo, chromosome, Hanwoo
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